The extrinsic polypeptides of Photosystem II
نویسندگان
چکیده
منابع مشابه
Mass spectroscopy locates the extrinsic proteins of photosystem II.
The photosynthetic oxidation of water is catalyzed by photosystem II (PSII), a multisubunit pigment-protein embedded in the thylakoid membranes of cyanobacteria, algae, and plants. This remarkable photoenzyme is capable of generating the highly oxidizing chemical species required to extract four tightly bound electrons of two substrate water-molecules, yielding biosynthetically useful reductant...
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Photosystem II, Active Particles, Polypeptides, Thylakoid Membrane, Anacystis A thylakoid membrane preparation isolated from the blue-green alga Anacystis nidulans was freed from carboxysomes, soluble enzymes and the pigment P750 by floating in a discontinuous sucrose density gradient. In a buffer containing sucrose and the zwitterionic detergent Miranol S2M-SF the thylakoids were loaded on a l...
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One form of carbonic anhydrase (CA) has been observed in maize (Zea mays) thylakoids and photosystem II (PSII)-enriched membranes. Here, we show that an antibody produced against a thylakoid lumen-targeted CA found in Chlamydomonas reinhardtii reacts with a single 33-kD polypeptide in maize thylakoids. With immunoblot analysis, we found that this single polypeptide could be identified only in m...
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Synthesis and assembly of photosystems (PS) I and II polypeptides in etiochloroplasts isolated from greening wheat (Triticum aestivum L. cv Norin 61) seedlings were studied. The isolated etiochloroplasts synthesized PSI polypeptides of 66 and 15 kilodaltons, PSII polypeptides of 46 and 42 kilodaltons, and atrazine-binding 34 to 32 kilodalton polypeptide. Their assembly processes in the thylakoi...
متن کاملSurface Charge Density Changes in Isolated Photosystem II Membranes Induced by Depletion of the Extrinsic Polypeptides of the Oxygen Evolving System
Treatment of PS II particles with either 1 m NaCl or alkaline Tris (1 m , pH 8.4) caused a considerable decrease in the average net negative surface charge density, concomitant with de pletion of the extrinsic 17, 24 and 33 kDa proteins of the oxygen evolving complex from the membranes. The partial recovery of the values for surface charge in both NaCland Tris-treated membranes was registered ...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 1996
ISSN: 0005-2728
DOI: 10.1016/s0005-2728(96)00102-8